Researchers have found a way to monitor changes and observe ion fluxes by studying the behaviour of the water molecules surrounding the membranes of neurons.
A team of researchers at the Laboratory for fundamental BioPhotonics (LBP) within EPFL‘s School of Engineering, Switzerland, have come up with a way to track the changes in membrane potential and to observe ion fluxes by investigating the behaviour of the water molecules surrounding membranes.
Neurons are brain cells that communicate with each other by sending electrochemical signals along axons. When a neuron is about to release a signal – in the form of an electric charge – it allows ions to pass through its membrane via ion channels. This ion transfer creates an electrical potential difference between the inside and outside of the cell, and that difference is referred to as the membrane potential.
The end of electrodes & fluorophores
Up until now, the only way to monitor neurons was by injecting fluorophores into, or attaching electrodes onto, the part of the brain being studied – but fluorophores can be toxic, and electrodes can damage the neurons.
The LBP researchers developed a way of tracking electrical movement in neurons by looking at the interactions between water molecules and the neural membranes.
Sylvie Roke, director of the LBP explains: “Neurons are surrounded by water molecules, which change orientation in the presence of an electric charge.”
“When the membrane potential changes, the water molecules will re-orient – and we can observe that.”
A better understanding of the electrical activity of neurons could provide insight into a number of processes taking place in our brains. For example, scientists could see whether a neuron is active or resting, or if it is responding to drug treatment.
Altering the neuronal membrane
In their study, the researchers altered the neuronal membrane potential by subjecting the neurons to a rapid influx of potassium ions. This caused the ion channels on the neurons’ surface – which serve to regulate the membrane potential – to open and let the ions through. The researchers then turned off the flow of ions, and the neurons released the ions that they had picked up.
In order to monitor this activity, the researchers probed the hydrated neuronal lipid membranes by illuminating the cells with two laser beams of the same frequency. These beams consist of femtosecond laser pulses -using technology for which the 2018 Nobel prize in physics was awarded- so that the water molecules on the interface of the membrane generate photons with a different frequency, known as second-harmonic light.
Roke concludes: “We see both fundamental and applied implications of our research. Not only can it help us understand the mechanisms that the brain uses to send information, but it could also appeal to pharmaceutical companies interested in in vitro product testing.”